A protein of around 58 kDa was recognized in the sample from affinity-purified proteins with biotin-conjugated macroketone specifically, however, not in the sample with free of charge biotin (Fig. with free of charge biotin. Strepavidin conjugated agarose beads had been added. After intensive washes, certain proteins were solved and eluted by SDS-PAGE. A protein of … Continue reading A protein of around 58 kDa was recognized in the sample from affinity-purified proteins with biotin-conjugated macroketone specifically, however, not in the sample with free of charge biotin (Fig